Latent infection of CD4+ T cells provides a mechanism for lifelong persistence of HIV-1, even in patients on effective combination therapy. Diana Finzi, Joel N. Combination therapy for HIV-1 infection can reduce plasma virus to Thus, latent infection of resting CD4+ T cells provides a mechanism for lifelong persistence Diana Finzi, Joel Blankson, +14 authors Robert F. Siliciano; Published in. Due to the importance of the latent reservoir in maintaining infection despite .. the long-term persistence of latent virus in HIV-infected individuals Finzi et al.

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Studies of the kinetics of the free virus turnover in the plasma of HIV-1—infected adults and children who are treated with highly active antiretroviral therapy HAART have shown similar decay patterns. However, the data presented here indicate that the latent reservoir for HIV-1 is established in infected children and will likely represent a major barrier to virus eradication that will have to be considered in all therapeutic strategies for the treatment of pediatric HIV-1 infection.

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Kinetics of response in lymphoid tissues to antiretroviral therapy of HIV-1 infection. Sequence validation was carried out by recommended methods Dornadula G, et al. Positions in protease at which polymorphisms occur are shown in black type. Culture assay for latently infected cells.

Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection.

Open in a separate window. Showing of 38 references. Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy. Skip to search form Skip to main content.

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A stable latent reservoir for HIV-1 in resting CD4+ T lymphocytes in infected children

Citations Publications citing this paper. Los Alamos National Laboratory. Bukrinsky MI, et al. However 2 of the 4 isolates contained amino acid insertions at position 69 [69 Ser- Ser-Val ] reported to occur with treatment with zidovudine in ihfection with didanosine or zalcitabine and associated with high-level resistance to the nucleoside RT inhibitors 2939 — Highly active antiretroviral therapy.


Sequencing of the HIV-1 pol gene. Informed consent was obtained from the parent or guardian for all study subjects, and assent was obtained from those children who were aware of their diagnosis.

Chun TW, et al.

The preintegration form of HIV-1 latency is relatively unstable, with an estimated half-life as short as 6 days J. In cases where all determinations were negative, an upper bound on the infected cell frequency was estimated by making the conservative assumption that the next highest cell concentration would be positive.

Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection.

Three additional children patients 2, 8, and 12who at entry had been receiving HAART for 4, 16, and 8 months, respectively, and who had sustained suppression of viral replication to undetectable levels, were also followed longitudinally for 17—21 months. Viral measurement by polymerase chain reaction-based assays in human immunodeficiency virus-infected infants. The median age at entry was 7. Please review our privacy policy.

Larder BA, et al. Observed resistance mutations were consistent with treatment history of infedtion patients. Link to citation list in Scopus. The precise mechanisms involved in the generation of this latent reservoir are unknown; the occasional reversion of HIV-1—infected, activated T lymphocytes to a resting memory state with integrated HIV-1 is one plausible mechanism.

Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection. In these 4 children, within 1 to 8 months of therapy with HAART it became difficult to culture HIV-1 at 1 or more time points using the standard input of 2. Numbers in the top row indicate RT codons associated with infwction resistance and protease codons associated with drug resistance, common polymorphisms, or polymorphism and accessory substitutions to protease inhibitors.


Children with perinatally acquired HIV-1 infection were eligible.

Sequencing was performed on viral isolates that represent distinct biological clones obtained with a limiting dilution culture technique. Montaner The Journal of infectious diseases This child was treated with nonsuppressive antiretroviral regimens consisting of zidovudine, lamivudine, didanosine, and zalcitabine for 3. Combination therapy for HIV-1 infection can reduce plasma virus to undetectable levels, indicating that prolonged treatment might eradicate the infection.

Thus, there was no decay in this reservoir despite prolonged suppression of viral replication. Known polymorphisms infectiion are also seen as accessory substitutions contributing to resistance to ritonavir RTV or nelfinavir NFV are shown in colored type. Revised classification system for human immunodeficiency virus in children less than 13 years of age. Onfection positions of the primers are numbered katent to the pol gene of the HXB2R isolate http: The reverse transcriptase codon 69 insertion is observed in nucleoside reverse transcriptase inhibitor-experienced HIVinfected individuals, including those without prior or concurrent zidovudine therapy.

Recombination favors the evolution of drug resistance in HIV-1 during antiretroviral therapy. Viral load and disease progression in infants infected with human immunodeficiency virus type 1.